Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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3,106 – 3,120 of 3,489 results

3,106

Alkali Scientific PIPES Sesquisodium Salt [Piperazine N,N' bis (2 ethanesulfonic acid) sesquisodium salt], 5 Kilograms

PIPES Sesquisodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sesquisodium salt), 5 Kilograms, is a highly specialized biochemical reagent used primarily for preparing buffers in laboratory research. PIPES is particularly valuable for maintaining a stable pH in biological systems, including enzyme reactions and cell culture environments. It is commonly used in electrophoresis, chromatography, and as a component in molecular biology protocols. The large 5 kg volume makes it ideal for high-throughput laboratories and large-scale biochemical experiments. PIPES is known for its exceptional buffering capacity in the physiological pH range, making it an essential tool in many laboratory applications.

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3,107

Medchemexpress LLC Glycine, N-(2-amino-2-oxoethyl)-N-(carboxymethyl)-, sodium salt (1:2) | 41689-31-0 | 98.0% | 25 G

ADA disodium is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers.

Derived from adenosine deaminase
Used in biochemical research
Ideal for enzyme buffer preparation

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3,108

LICORbio Odyssey MES SDS Running Buffer Powder (pack of 5)

Odyssey Mini Gels are polyacrylamide gels designed to separate a wide range of protein sizes via PAGE or SDS-PAGE. Each box contains 10 pre-cast gels. Our gels feature larger well volumes for easier sample loading and are cast in a neutral pH buffer to increase gel stability. They also run at neutral pH, which minimizes protein modification compared to Tris-glycine gels. Odyssey Mini Gels are available in several gradients with 10, 12, or 15 numbered wells. Our gels feature a shelf life of up to 6 months at room temperature and 12 months at 2–8C.

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3,109

Dojindo Molecular Technologies Inc TAPS (25G), Good's buffer, CAS: 29915-38-6, pka=8.4, Active pH 7.7-9.1, made in Japan

In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.

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3,110

eMolecules 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 20656-08-0 | MFCD00141691 | 1g

Matrix Scientific | 2,4-Dichloro-N-(2-hydroxyethyl)benzenecarboxamide | 1g | 389721058 | 45569 | 95.000 | 20656-08-0 | MFCD00141691 | 234.080 | C9H9Cl2NO2

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3,111

Teknova 50mM MES 1M Sodium Chloride

50mM MES, 1M Sodium Chloride, pH 6.0, 1000mL, Endotoxin Tested, Sterile. 8 pack.

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3,112

Cambridge Isotope Laboratories PIPES (D18 98%) 0 25 g

PIPES (D18 98%) 0 25 g

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3,113

Alkali Scientific MOPSO [3 (N Morpholino) 2 hydroxypropanesulfonic acid], 100 G

MOPSO (3-(N-Morpholino)2-hydroxypropanesulfonic acid) sodium salt is a specialized pH buffer used in biochemical applications where precise pH control is necessary. This 100g volume of MOPSO sodium salt is ideal for laboratories that require small quantities of this specific buffer for experiments that involve enzymatic reactions, protein assays, or cell culture applications. MOPSO offers a high degree of buffering capacity in the pH range of 7.0–7.5, making it suitable for a variety of molecular biology protocols. Its stability at this pH range is ideal for experiments where small fluctuations in pH can significantly affect the outcome. MOPSO is particularly valuable in applications that require precise control of the pH environment.

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3,114

Bioworld ADA (N-(2-acetamido)iminodiacetic acid) Buffer, 0.2M, pH 7.0, 250 mL

Synonym:ADA-Na,N-(2-Acetamido)iminodiacetic acid monosodium salt,N-(Carbamoylmethyl)iminodiacetic acid monosodium saltA zwitterionic buffer used in biological applications including protein isolation and characterizationWarning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption. Appearance: LiquidPH Value: 7.0

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3,115

Medchemexpress LLC IMINODIACETIC ACID 10MM 1ML | 10MM/1ML

IMINODIACETIC ACID 10MM 1ML | 10MM/1ML

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3,116

Alkali Scientific MOPSO Sodium Salt, 500 G

MOPSO Sodium Salt, 500 G, is a high-quality buffering reagent used to stabilize pH in laboratory experiments, particularly those involving proteins, nucleic acids, and enzyme reactions. MOPSO is known for its ability to maintain a stable pH in the range of 6.5 to 7.9, which is essential for many biochemical assays and protocols. This larger 500 g package is ideal for high-volume or repeated experiments and provides a cost-effective solution for laboratories working on large-scale studies or industrial applications. MOPSO is widely used in cell culture, protein analysis, and other critical research areas requiring reliable pH control.

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3,117

Alkali Scientific TAPS, [Tris(hydroxymethyl) 3 aminopropanesulfonic acid], Free Acid, 1 Kg

TAPS, or Tris(hydroxymethyl) aminopropanesulfonic acid, is a high-purity buffering agent provided in the free acid form for use in laboratory settings. This 1kg quantity is perfect for large-scale buffer preparation, particularly in molecular biology, electrophoresis, and protein studies. TAPS helps maintain a stable pH in solutions, making it ideal for a variety of biological assays, including enzyme reactions and chromatography. The high purity of this reagent ensures that it will not interfere with sensitive experiments, delivering consistent and reproducible results. The 1kg size offers cost-effective, long-term use for labs that regularly prepare TAPS-based buffers.