Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Mitegen Bis-Tris/ HCl, 1M, pH5.2, 250 ml, CAS # 6976-37-0
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Sigma Aldrich Fine Chemicals Biosciences Trizma Pre-set crystals, 100 g
BioPerformance Certified, pH 8.0, average Mw 141.8, Synonym: Tris crystals. The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt BioPerform100G
MES is one of the so-called Good buffers developed for biological applications. This product is BioPerformance certified (suitable for electrophoresis, tissue culture, and molecular biology applications. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa/-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
STEMCELL Technologies HEPES Buffer Solution (1 M), Size: 100 mL
HEPES Buffer Solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.
Nusep Inc NUVIEW MINI GEL 4-20 12-WELL
NuSep's nUView Tris-Glycine SDS-PAGE Precast Gel 4-20% 12well. Cassette size: 10 x 8.5 cm. Fits Mini-PRO or Bio-Rad's Mini-Protean Tetra cell Tanks. 10 gel cassettes per box. All nUView™ precast gels incorporate a unique formulation allowing protein bands to be visualized in only 2 minutes in the presence of ultraviolet (UV) light. Simply place your gel over a 250-320 nm UV trans-illuminator and watch high-resolution bands appear in just 2 minutes. With nUView™ technology you no longer have to stain and de-stain your gels – increasing protein recovery and saving you significant time while receiving improved results.
Apexbio Technology LLC EdU Flow Cytometry Assay Kits (488) 100 tests
EdU Flow Cytometry Assay Kits (488) provides a novel method based on click chemistry specifically CuAAC (copper-catalyzed azide-alkyne cycloaddition) enabling direct measurement of DNA synthesis during the S-phase of the cell cycle The thymidine analog EdU (5-ethynyl-2 -deoxyuridine) can be incorporated directly into DNA strands during DNA synthesis The alkyne group on EdU a biologically inert moiety undergoes highly selective reaction with azide-functionalized dyes via the CuAAC reaction forming a stable 1 2 3-triazole product The unique biological groups on EdU and 6-FAM Azide allow fluorescent labeling of proliferating cell DNA after conjugation enabling imaging with low background and high detection sensitivity The EdU Flow Cytometry Assay Kits (488) use 6-FAM azide to label the DNA of proliferating cells following its reaction with EdU Samples labeled in this manner can be analyzed via flow cytometry (EXC/EMI 496/516 nm)) with green fluorescence
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula III Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Electron Microscopy Sciences D-PBS, Formula II Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Teknova 50mM MES, pH 6.0, 1000mL, Endotoxin Tested, Sterile.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.